| Viro Dynamics offers complete Immunochemistry services, from antigen development to antibody purification and packaging. Conjugation of Antigen Conjugation to KLH, BSA, OVA. Other carriers also available. Terminal modifications. C-Terminal amide formation and N-Terminal acetylation. Purification of IgG IgG will be purified from serum or ascites fluid using protein G chromatography. Protein concentration will be determined. Final yield of purified antibodies depends on titer. Affinity Purification of Antibodies PURIFICATION OF ANTIBODIES FROM 10-20ML SERUM Covalently link peptide or protein (1-2mg) to column through amino groups Antibody Titration by ELISA TITRATION ASSAY IN 96 WELL MICROPLATE ASSAY PERFORMED IN DUPLICATE FOUR SERA CAN BE TITERED PER PLATE 10 to 20 ug antigen needed per plate for coating protein concentration must be provided need to know buffer conditions (any detergent) Western Blot Assay FOUR LANES ASSAYED need to supply 2 to 5 ug/lane protein concentration must be provided need to know buffer conditions (any detergent) approximate molecular weight of protein of interest SDS Polyacrylamide Gel Electophoresis FOUR LANES ASSAYED SAMPLE RUN UNDER REDUCING CONDITIONS COOMASSIE STAINED GELS need to supply 50 to 100 ug/lane protein concentration must be provided need to know buffer conditions (any detergent) approximate molecular weight of protein of interest Purification of IgY IgY will be purified from twelve eggs by polyethylene glycol precipitation. Protein concentration will be determined. |
| Immunochemistry Services |
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